Cryoprotectant kinetic analysis of a human articular cartilage vitrification protocol

L-proline: a highly effective cryoprotectant for mouse oocyte vitrification

Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...

Computerized Morphometric Analysis of Human Femoral Articular Cartilage

Objective. Articular cartilage shows changes with age that are considered to be the most important factors in the development and progression of osteoarthritis. The studies on age changes in articular cartilage have been traditionally based on individual observations but this approach is limited by its subjectivity and bias, yielding considerable variability. So the present study was conducted ...

Differential Immunohistochemical Expression Pattern of Galectin-3 in Normal and Osteoarthritic Human Articular Cartilage

Background: Previous studies have shown that Galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones.   Objective: In the present work, the expression pattern of Galectin-3 in normal and various grades of osteoarthritic (OA) human articular cartilage has been studied.   Methods: Using immunohistochemistry and standard we...

Electrokinetic evaluation of human articular cartilage

The reactions of injured human articular cartilage.

The physical and chemical structure of normal hyaline articular cartilage was described by Collins (1949), but there is little published on its reactions to injury and repair in the human subject, though animal observations have been made, notably by Bennett, Bauer and Maddock (1932). The slow metabolism and physiological inactivity of joint cartilage were emphasised by Bywaters (1937), again m...